Therapy of intracellular Staphylococcus aureus by tigecyclin

Background: In the fields of traumatology and orthopaedics staphylococci are the most frequently isolated pathogens. Staphylococcus aureus and Staphylococcus epidermidis are known to be the major causative agents of osteomyelitis. The increasing number of multiresistant Staphylococcus aureus and res...

Authors: Kreis, Carolin A.
Raschke, Michael J.
Roßlenbroich, Steffen Bernd
Tholema-Hans, Nancy
Löffler, Bettina
Fuchs, Thomas
Division/Institute:FB 05: Medizinische Fakultät
Document types:Article
Media types:Text
Publication date:2013
Date of publication on miami:21.02.2014
Modification date:26.04.2019
Edition statement:[Electronic ed.]
Source:BMC Infectious Diseases 13 (2013) 367
Subjects:Osteomyelitis; Implant associated infection; Staphylococcus aureus; Tigecyclin; Biomechanical stability
DDC Subject:610: Medizin und Gesundheit
License:CC BY 2.0
Language:English
Notes:Finanziert durch den Open-Access-Publikationsfonds 2013/2014 der Deutschen Forschungsgemeinschaft (DFG) und der Westfälischen Wilhelms-Universität Münster (WWU Münster).
Format:PDF document
URN:urn:nbn:de:hbz:6-24319434022
Other Identifiers:DOI: 10.1186/1471-2334-13-267
Permalink:https://nbn-resolving.de/urn:nbn:de:hbz:6-24319434022
Digital documents:1471-2334-13-267.pdf

Background: In the fields of traumatology and orthopaedics staphylococci are the most frequently isolated pathogens. Staphylococcus aureus and Staphylococcus epidermidis are known to be the major causative agents of osteomyelitis. The increasing number of multiresistant Staphylococcus aureus and resistant coagulase-negative staphylococci as a trigger of complicated osteomyelitis and implant-associated infections is a major problem. Antibiotic therapy fails in 20% of cases. Therefore the development of novel antibiotics becomes necessary. Methods: This study analyses tigecyclin, the first antibiotic of the glycylines, as a potential therapy for osteomyelitis caused by multiresistant Staphylococcus aureus. Therefore its intracellular activity and the potential use in polymethylmetacrylate-bone cement are examined. The intracellular activity of tigecyclin is determined by a human osteoblast infection model. The investigation of the biomechanical characteristics is conducted concerning the ISO 5833-guidelines. Results: Tigecyclin shows in vitro an intracellular activity that ranges between the antimicrobial activity of gentamicin and rifampicin. A significant negative effect on the biomechanical characteristics with an impaired stability is detected after adding tigecyclin to polymethylmetacrylate-bone cement with a percentage of 1.225% per weight. Conclusions: This study shows that tigecyclin might be a potent alternative for the systemic therapy of osteomyelitis and implant-associated infections whereas the local application has to be reconsidered individually.