Does pre-incubation in selective-enrichment media improve the detection of diarrheagenic Escherichia coli using the RIDA®GENE PCR?
We aimed to investigate whether a selective pre-PCR enrichment step improves test performance of RIDA®GENE EHEC/EPEC to detect diarrheagenic 'Escherichia coli' from stool samples. Each of the 250 stool samples was analyzed for the presence of stx1/2 and eae both with and without pre-PCR en...
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| FB/Einrichtung: | FB 05: Medizinische Fakultät |
| Dokumenttypen: | Artikel |
| Medientypen: | Text |
| Erscheinungsdatum: | 2023 |
| Publikation in MIAMI: | 14.11.2023 |
| Datum der letzten Änderung: | 14.11.2023 |
| Angaben zur Ausgabe: | [Electronic ed.] |
| Quelle: | International Journal of Medical Microbiology 313 (2023) 2, 151575, 1-4 |
| Schlagwörter: | Enteropathogenic Escherichia coli; Enterohemorrhagic Escherichia coli; Culture; Enrichment; polymerase chain reaction |
| Fachgebiet (DDC): | 610: Medizin und Gesundheit |
| Lizenz: | CC BY 4.0 |
| Sprache: | English |
| Förderung: | Finanziert durch den Open-Access-Publikationsfonds der Universität Münster. |
| Format: | PDF-Dokument |
| URN: | urn:nbn:de:hbz:6-28958663928 |
| Weitere Identifikatoren: | DOI: 10.17879/68958455097 |
| Permalink: | https://nbn-resolving.de/urn:nbn:de:hbz:6-28958663928 |
| Verwandte Dokumente: |
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| Onlinezugriff: | 10.1016_j.ijmm.2023.151575.pdf |
We aimed to investigate whether a selective pre-PCR enrichment step improves test performance of RIDA®GENE EHEC/EPEC to detect diarrheagenic 'Escherichia coli' from stool samples. Each of the 250 stool samples was analyzed for the presence of stx1/2 and eae both with and without pre-PCR enrichment in selective broth. In comparison to a reference method, sensitivities for stx1/2 and eae with and without pre-PCR enrichment were 84% (95%CI 70–93) and 89% (stx1/2, 95%CI 76–96), and 71% (95%CI 58–81) and 72% (eae, 95%CI 60–82), respectively. Specificity exceeded 97% for both methods and target genes. In summary, pre-PCR broth enrichment did not improve test performance.