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In vitro inhibition of HUVECs by low dose methotrexate - Insights into oral adverse events

Background: With socio-economic changes, dentists and maxillofacial surgeons are more and more faced with medically compromised patients. Especially, the admission of antirheumatic drugs has increased remarkably. So dentists and maxillofacial surgeons should be aware of related adverse reactions that affect the craniofacial region. To identify possible cellular effects of disease modifying antirheumatic drugs (DMARDs) we investigated the influence of methotrexate (MTX) on human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were incubated with various concentrations of MTX, corresponding to serum concentrations found in rheumatoid arthritis (RA) patients. The effect of MTX on cell proliferation, differentiation as well as mitochondrial activity was measured by use of immunostaining, cell counting and 3-(4, 5-dimethylthiazol-2-yl)- 2, 5-diphenyltetrazolium bromide (MTT) assay. Results: All samples incubated with MTX (1-1000 nM) showed significantly decreased cell viability when compared to controls. Cells were less proliferating, but did not lose their ability to synthesize endothelial proteins. A slight dose dependency of inhibiting effects was demonstrated. The observed differences between control and sample groups were rising with longer duration. Conclusion: Because of the crucial role of endothelial cells and their precursor cells in wound healing, a negative influence of MTX on oral health has to be supposed, correlating to clinical observations of adverse reactions in the oral cavity, such as ulcerative or erosive lesions.

Titel: In vitro inhibition of HUVECs by low dose methotrexate - Insights into oral adverse events
Verfasser: Annussek, Tobias GND
Szuwart, Thomas GND
Kleinheinz, Johannes GND
Koiky, Cathrin GND
Wermker, Kai GND
Organisation: FB 05: Medizinische Fakultät
Dokumenttyp: Artikel
Medientyp: Text
Erscheinungsdatum: 22.05.2014
Publikation in MIAMI: 20.11.2014
Datum der letzten Änderung: 16.04.2019
Quelle: Head & Face Medicine 10 (2014) 19, 1-7
Schlagwörter: Oral health; Antirheumatic drugs; Methotrexate; Endothelial cells; In vitro; Wound healing
Fachgebiete: Medizin und Gesundheit
Lizenz: CC BY 2.0
Sprache: Englisch
Anmerkungen: Finanziert durch den Open-Access-Publikationsfonds 2014/2015 der Deutschen Forschungsgemeinschaft (DFG) und der Westfälischen Wilhelms-Universität Münster (WWU Münster).
Format: PDF-Dokument
URN: urn:nbn:de:hbz:6-01349481116
Permalink: https://nbn-resolving.org/urn:nbn:de:hbz:6-01349481116
DOI: doi:10.1186/1746-160X-10-19
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