Cytotoxic effects of four different root canal sealers on human osteoblasts
The aim of this study was to evaluate the effect of an epoxy resin-based (AH-Plus), a zinc oxide eugenol containing (Pulp-Canal-Sealer) and two calcium silicate containing (MTA-Fillapex and BioRoot-RCS) sealers on primary human osteoblasts (hOB) in freshly mixed and set state. All sealers were mixed...
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FB/Einrichtung: | FB 05: Medizinische Fakultät |
Dokumenttypen: | Artikel |
Medientypen: | Text |
Erscheinungsdatum: | 2018 |
Publikation in MIAMI: | 03.04.2018 |
Datum der letzten Änderung: | 31.05.2022 |
Angaben zur Ausgabe: | [Electronic ed.] |
Quelle: | PLoS ONE 13 (2018) 3, e0194467, 1-14 |
Schlagwörter: | AH Plus; BioRoot RCS; cytotoxicity; MTA Fillapex; Pulp Canal Sealer; Osteoblast |
Fachgebiet (DDC): | 610: Medizin und Gesundheit |
Lizenz: | CC BY 4.0 |
Sprache: | Englisch |
Format: | PDF-Dokument |
URN: | urn:nbn:de:hbz:6-79109502945 |
Weitere Identifikatoren: | DOI: 10.1371/journal.pone.0194467 |
Permalink: | https://nbn-resolving.de/urn:nbn:de:hbz:6-79109502945 |
Onlinezugriff: | artikel_dammaschke_2018.pdf |
The aim of this study was to evaluate the effect of an epoxy resin-based (AH-Plus), a zinc oxide eugenol containing (Pulp-Canal-Sealer) and two calcium silicate containing (MTA-Fillapex and BioRoot-RCS) sealers on primary human osteoblasts (hOB) in freshly mixed and set state. All sealers were mixed strictly according to the manufacturers´ instructions and identically samples were produced. In a pretest cytotoxic sealer concentrations were determined. Thus, for the main cell culture study, dilutions of sealer extract 1:1, 1:2, and 1:10 were used. To simulate a clinical scenario, extracts from freshly mixed sealer were added to the cells on day one. Extracts form set sealers were used for subsequent culturing for 24h, 7d, 14d, and 21d. Cell viability was analyzed by living-cell-count, MTT-assay, and living/dead-staining, cytotoxicity by LDH-assay, and changes by Richardson-staining. All data were statistically evaluated by one way ANOVA and a posthoc analysis with Bonferroni-Holm testing (p