Cytotoxic effects of four different root canal sealers on human osteoblasts

The aim of this study was to evaluate the effect of an epoxy resin-based (AH-Plus), a zinc oxide eugenol containing (Pulp-Canal-Sealer) and two calcium silicate containing (MTA-Fillapex and BioRoot-RCS) sealers on primary human osteoblasts (hOB) in freshly mixed and set state. All sealers were mixed...

Authors: Jung, Susanne
Sielker, Sonja
Hanisch, Marcel
Libricht, Viktor
Schäfer, Edgar
Dammaschke, Till
Division/Institute:FB 05: Medizinische Fakultät
Document types:Article
Media types:Text
Publication date:2018
Date of publication on miami:03.04.2018
Modification date:31.05.2022
Edition statement:[Electronic ed.]
Source:PLoS ONE 13 (2018) 3, e0194467, 1-14
Subjects:AH Plus; BioRoot RCS; cytotoxicity; MTA Fillapex; Pulp Canal Sealer; Osteoblast
DDC Subject:610: Medizin und Gesundheit
License:CC BY 4.0
Language:English
Format:PDF document
URN:urn:nbn:de:hbz:6-79109502945
Other Identifiers:DOI: 10.1371/journal.pone.0194467
Permalink:https://nbn-resolving.de/urn:nbn:de:hbz:6-79109502945
Digital documents:artikel_dammaschke_2018.pdf

The aim of this study was to evaluate the effect of an epoxy resin-based (AH-Plus), a zinc oxide eugenol containing (Pulp-Canal-Sealer) and two calcium silicate containing (MTA-Fillapex and BioRoot-RCS) sealers on primary human osteoblasts (hOB) in freshly mixed and set state. All sealers were mixed strictly according to the manufacturers´ instructions and identically samples were produced. In a pretest cytotoxic sealer concentrations were determined. Thus, for the main cell culture study, dilutions of sealer extract 1:1, 1:2, and 1:10 were used. To simulate a clinical scenario, extracts from freshly mixed sealer were added to the cells on day one. Extracts form set sealers were used for subsequent culturing for 24h, 7d, 14d, and 21d. Cell viability was analyzed by living-cell-count, MTT-assay, and living/dead-staining, cytotoxicity by LDH-assay, and changes by Richardson-staining. All data were statistically evaluated by one way ANOVA and a posthoc analysis with Bonferroni-Holm testing (p