Specific role of collagen cross-linking enzymes (lysyl oxidase and tissue transglutaminase) in supramolecular organization of matrix in chicken embryonic cornea and tendon

Mature cornea consists of orthogonally stacked lamellae formed by uniformly thin collagen fibrils evenly spaced in parallel. In tissue, collagens must be cross-linked to exhibit normal physical properties. In the extracellular matrix (ECM), cross-linking can result from the enzymatic activity of lys...

Author: Wang, Lei
Further contributors: Bruckner, Peter (Thesis advisor)
Division/Institute:FB 13: Biologie
FB 05: Medizinische Fakultät
Document types:Doctoral thesis
Media types:Text
Publication date:2011
Date of publication on miami:28.12.2011
Modification date:06.06.2016
Edition statement:[Electronic ed.]
Subjects:Cornea; extrazelluläre Matrix; Cross-Link; Lysyl-Oxidase; Transglutaminase
DDC Subject:570: Biowissenschaften; Biologie
License:InC 1.0
Language:English
Format:PDF document
URN:urn:nbn:de:hbz:6-91499580787
Permalink:https://nbn-resolving.de/urn:nbn:de:hbz:6-91499580787
Digital documents:diss_wang.pdf

Mature cornea consists of orthogonally stacked lamellae formed by uniformly thin collagen fibrils evenly spaced in parallel. In tissue, collagens must be cross-linked to exhibit normal physical properties. In the extracellular matrix (ECM), cross-linking can result from the enzymatic activity of lysyl oxidase (LOX) and tissue transglutaminase (TG). The relationship between tissue-specific matrix organization and cross-links in the ECM of chicken embryonic cornea has been investigated. We found that typical sheets of orthogonally arranged collagen fibrils were formed by keratocyte cell cultures. Such lamellae were not apparent when aldehyde-derived cross-link formation was inhibited. However, the collagen fibrils formed were thicker with a clearly visible banding pattern with the TG inhibitor. Interestingly, the presence of both cross-link leads to striking effects on matrix formation visible by loss of matrix organization and an irregular fibril diameter distribution.