Phenotypic and Genotypic Characterization of 'Escherichia coli' Causing Urinary Tract Infections in Kidney-Transplanted Patients

Urinary tract infection (UTI), frequently caused by uropathogenic Escherichia coli (UPEC), is the most common infection after kidney transplantation (KTx). Untreated, it can lead to urosepsis and impairment of the graft function. We questioned whether the UPEC isolated from KTx patients differed fro...

Authors: Abo Basha, Jonas
Kiel, Matthias
Görlich, Dennis
Schütte-Nütgen, Katharina
Witten, Anika
Pavenstädt, Hermann
Kahl, Barbara C.
Dobrindt, Ulrich
Reuter, Stefan Johannes
Division/Institute:FB 05: Medizinische Fakultät
Document types:Article
Media types:Text
Publication date:2019
Date of publication on miami:03.07.2020
Modification date:03.07.2020
Edition statement:[Electronic ed.]
Source:Journal of Clinical Medicine 8 (2019) 7, 988, 1-14
Subjects:Uropathogenic E. coli; UPEC; phylogeny; genomics; antibiotic resistance; virulence traits; kidney transplantation
DDC Subject:610: Medizin und Gesundheit
License:CC BY 4.0
Language:English
Funding:Finanziert durch den Open-Access-Publikationsfonds der Westfälischen Wilhelms-Universität Münster (WWU Münster).
Format:PDF document
URN:urn:nbn:de:hbz:6-30189562423
Permalink:http://nbn-resolving.de/urn:nbn:de:hbz:6-30189562423
Other Identifiers:DOI: 10.3390/jcm8070988
Digital documents:artikel_reuter_2019.pdf
zusatzmaterial_reuter_2019.pdf
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Urinary tract infection (UTI), frequently caused by uropathogenic Escherichia coli (UPEC), is the most common infection after kidney transplantation (KTx). Untreated, it can lead to urosepsis and impairment of the graft function. We questioned whether the UPEC isolated from KTx patients differed from the UPEC of non-KTx patients. Therefore, we determined the genome sequences of 182 UPEC isolates from KTx and control patients in a large German university clinic and pheno- and genotypically compared these two isolated groups. Resistance to the β-lactams, trimethoprim or trimethoprim/sulfamethoxazole was significantly higher among UPEC from KTx than from control patients, whereas both the isolated groups were highly susceptible to fosfomycin. Accordingly, the gene content conferring resistance to β-lactams or trimethoprim, but also to aminoglycosides, was significantly higher in KTx than in control UPEC isolates. E. coli isolates from KTx patients more frequently presented with uncommon UPEC phylogroups expressing higher numbers of plasmid replicons, but interestingly, less UPEC virulence-associated genes than the control group. We conclude that there is no defining subset of virulence traits for UPEC from KTx patients. The clinical history and immunocompromised status of KTx patients enables E. coli strains with low uropathogenic potential, but with increased antibiotic resistance to cause UTIs.