A Plant Extract of Ribes nigrum folium Possesses Anti-Influenza Virus Activity In Vitro and In Vivo by Preventing Virus Entry to Host Cells

Infections with influenza A viruses (IAV) are still amongst the major causes of highly contagious severe respiratory diseases not only bearing a devastating effect to human health, but also significantly impact the economy. Besides vaccination that represents the best option to protect from IAV infe...

Authors: Ehrhardt, Christina
Dudek, Sabine Eva
Holzberg, Magdalena Hildegard
Urban, Sabine
Hrincius, Eike-Roman
Haasbach, Emanuel
Seyer, Roman
Lapuse, Julia
Planz, Oliver
Ludwig, Stephan
Division/Institute:FB 13: Biologie
Document types:Article
Media types:Text
Publication date:2013
Date of publication on miami:17.02.2014
Modification date:16.04.2019
Edition statement:[Electronic ed.]
Source:PLoS ONE 8 (2013) 5, e63657
DDC Subject:610: Medizin und Gesundheit
License:CC BY 3.0
Language:German
Notes:Finanziert durch den Open-Access-Publikationsfonds 2013/2014 der Deutschen Forschungsgemeinschaft (DFG) und der Westfälischen Wilhelms-Universität Münster (WWU Münster).
Format:PDF document
URN:urn:nbn:de:hbz:6-64319537376
Permalink:http://nbn-resolving.de/urn:nbn:de:hbz:6-64319537376
Other Identifiers:DOI: 10.1371/journal.pone.0063657
Digital documents:journal.pone.0063657.pdf

Infections with influenza A viruses (IAV) are still amongst the major causes of highly contagious severe respiratory diseases not only bearing a devastating effect to human health, but also significantly impact the economy. Besides vaccination that represents the best option to protect from IAV infections, only two classes of anti-influenza drugs, inhibitors of the M2 ion channel and the neuraminidase, often causing resistant IAV variants have been approved. That is why the need for effective and amply available antivirals against IAV is of high priority. Here we introduce LADANIA067 from the leaves of the wild black currant (Ribes nigrum folium) as a potent compound against IAV infections in vitro and in vivo. LADANIA067 treatment resulted in a reduction of progeny virus titers in cell cultures infected with prototype avian and human influenza virus strains of different subtypes. At the effective dose of 100 µg/ml the extract did not exhibit apparent harming effects on cell viability, metabolism or proliferation. Further, viruses showed no tendency to develop resistance to LADANIA067 when compared to amantadine that resulted in the generation of resistant variants after only a few passages. On a molecular basis the protective effect of LADANIA067 appears to be mainly due to interference with virus internalisation. In the mouse infection model LADANIA067 treatment reduces progeny virus titers in the lung upon intranasal application. In conclusion, an extract from the leaves of the wild black currant might be a promising source for the development of new antiviral compounds to fight IAV infections.