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|a urn:nbn:de:hbz:6-36059704190
|2 urn
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|a eng
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|a 572 Biochemie
|2 23
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|a Aziz, Shahid
|4 aut
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|a Universitäts- und Landesbibliothek Münster
|0 http://d-nb.info/gnd/5091030-9
|4 own
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|a Albumin aggregation and the re-solubilization of dried serum proteins
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|a [Electronic ed.]
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|c 2021
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|b Universitäts- und Landesbibliothek Münster
|c 2021-09-06
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|a 1-3
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|a free access
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|a Albumin is known for its aggregation. As it is a major component of serum, it may be largely responsible for the difficulties in re-suspending dried serum proteins. We found it necessary to break up the solid pellet using a ball mill and subsequently a chaotropic agent and an organic solvent to generate a homogenous protein solution suitable for mass spectrometry-based protein expression analysis.
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|a Albumin ist bekannt für seine Aggregation. Da es eine Hauptkomponente von Serum darstellt, ist es wahrscheinlich hauptsächlich für die Schwierigkeiten bei der Resuspendierung getrockneter Serum-Proteine zuständig. Wir stellten fest, dass es notwendig war, zunächst das trockene Pellet mit einer Kugelmühle zu zerstören. Danach mussten Puffer mit Chaotrop und organischen Lösungsmittel zugefügt werden, um eine homogene Proteinlösung generieren, die für die Massenspektrometrie-basierte Expressionsanalyse geeignet war.
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|a specialized
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|a This investigation was part of a research study financially supported by the Pakistan Science Foundation (project # PSF/res/C-PINSTECH/Med (497)) and a fellowship from the International Research Support Initiative Program, Higher Education Commission, Pakistan, for SA.
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|a CC BY-SA 3.0 DE
|u http://creativecommons.org/licenses/by-sa/3.0/de/
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|a Albumin
|a serum
|a chaotropic agent
|a aggregation
|a liquid crystals
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|a Albumins
|a Serum
|a Chaotrop
|a Aggregation
|a Flüssigkristalle
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|2 DRIVER Types
|a Artikel
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|2 DCMI Types
|a Text
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|a Zahra, Rabaab
|4 aut
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|a Berg, Anna Maria
|4 aut
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|a Ackermann, Doreen
|u FB 05: Medizinische Fakultät
|0 http://d-nb.info/gnd/1016481713
|4 aut
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|a Rasheed, Faisal
|4 aut
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|a König, Simone
|4 aut
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|t Mercator Journal of Biomolecular Analysis
|g 5 (2021) 1, S. 1-3
|i IsPartOf
|w (miami)550225b9-b848-45d8-bec1-96a452c75b4b
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|3 Zum Volltext
|q text/html
|u https://nbn-resolving.de/urn:nbn:de:hbz:6-36059704190
|u urn:nbn:de:hbz:6-36059704190
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|3 Zum Volltext
|q application/pdf
|u https://repositorium.uni-muenster.de/document/miami/13c9386e-7f65-4324-a9b8-e9eb0c3375ba/mercator-journal_2021_5_1_1-3.pdf
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