U12-type Spliceosomal Introns of Insecta

Most of eukaryotic genes are interrupted by introns that need to be removed from pre-mRNAs before they can perform their function. This is done by complex machinery called spliceosome. Many eukaryotes possess two separate spliceosomal systems that process separate sets of introns. The major (U2) spl...

Authors: Janice, Jessin
Pande, Amit
Weiner, January
Lin, Chiao-Feng
Makałowski, Wojciech
Division/Institute:FB 13: Biologie
Document types:Article
Media types:Text
Publication date:2012
Date of publication on miami:12.03.2013
Modification date:27.01.2020
Edition statement:[Electronic ed.]
Source:International Journal of Biological Sciences 8 (2012) 3, 344-352
Subjects:U12-type introns; minor spliceosome; insect evolution
DDC Subject:570: Biowissenschaften; Biologie
License:CC BY-NC-ND 3.0 DE
Language:English
Notes:Finanziert durch den Open-Access-Publikationsfonds 2011/2012 der Deutschen Forschungsgemeinschaft (DFG) und der Westfälischen Wilhelms-Universität Münster (WWU Münster).
Format:PDF document
URN:urn:nbn:de:hbz:6-87359413828
Permalink:http://nbn-resolving.de/urn:nbn:de:hbz:6-87359413828
Other Identifiers:DOI: 10.7150/ijbs.3933
Digital documents:v08p0344.pdf

Most of eukaryotic genes are interrupted by introns that need to be removed from pre-mRNAs before they can perform their function. This is done by complex machinery called spliceosome. Many eukaryotes possess two separate spliceosomal systems that process separate sets of introns. The major (U2) spliceosome removes majority of introns, while minute fraction of intron repertoire is processed by the minor (U12) spliceosome. These two populations of introns are called U2-type and U12-type, respectively. The latter fall into two subtypes based on the terminal dinucleotides. The minor spliceosomal system has been lost independently in some lineages, while in some others few U12-type introns persist. We in-vestigated twenty insect genomes in order to better understand the evolutionary dynamics of U12-type introns. Our work confirms dramatic drop of U12-type introns in Diptera, leaving these genomes just with a handful cases. This is mostly the result of intron deletion, but in a number of dipteral cases, minor type introns were switched to a major type, as well. Insect genes that harbor U12-type introns belong to several functional categories among which proteins binding ions and nucleic acids are enriched and these few categories are also overrepresented among these genes that preserved minor type introns in Diptera.