Pseudo-MRM method for the selective detection of human HLA-G

A pseudo-MRM method using high-resolution mass spectrometry is presented for the specific detection of HLA-G against a background of homologous proteins. The method relies on three unique and two unspecific tryptic peptides. The latter are used to monitor the level of background proteins in comparis...

Authors: König, Simone
Spurny, Christian
Rössig, Claudia
Division/Institute:FB 05: Medizinische Fakultät
Document types:Article
Media types:Text
Publication date:2017
Date of publication on miami:02.11.2017
Modification date:02.11.2017
Source:Mercator Journal of Biomolecular Analysis, 1 (2017) 2, S. 12-21
Edition statement:[Electronic ed.]
Subjects:mass spectrometry; protein quantification; serum; immune checkpoint molecule; LC/MS
DDC Subject:572: Biochemie
License:CC BY-SA 3.0 DE
Language:English
Format:PDF document
URN:urn:nbn:de:hbz:6-00269469431
Permalink:http://nbn-resolving.de/urn:nbn:de:hbz:6-00269469431
Digital documents:mercator-journal_2017_1_2_12-21.pdf

A pseudo-MRM method using high-resolution mass spectrometry is presented for the specific detection of HLA-G against a background of homologous proteins. The method relies on three unique and two unspecific tryptic peptides. The latter are used to monitor the level of background proteins in comparison to the desired HLA-G. Instrument response is linear in the range from 30 to 200 fmol.

Eine pseudo-MRM-Methode mittels hochauflösender Massenspektrometrie für den spezifischen Nachweis von HLA-G in einem Untergrund von homologen Proteinen wird präsentiert. Die Methode nutzt drei eindeutige und zwei unspezifische tryptische Peptide. Letztere werden zur Einschätzung der Menge an homologen Proteinen verwendet. Die Messmethode ist linear im Bereich von 30-200 fmol.