Oral immune priming with 'Bacillus thuringiensis' induces a shift in the gene expression of 'Tribolium castaneum' larvae

BACKGROUND: The phenomenon of immune priming, i.e. enhanced protection following a secondary exposure to a pathogen, has now been demonstrated in a wide range of invertebrate species. Despite accumulating phenotypic evidence, knowledge of its mechanistic underpinnings is currently very limited. Here...

Authors: Greenwood, Jenny M.
Milutinović, Barbara
Peuß, Robert
Behrens, Sarah
Esser, Daniela
Rosenstiel, Philip
Schulenburg, Hinrich
Kurtz, Joachim
Division/Institute:FB 13: Biologie
Document types:Article
Media types:Text
Publication date:2017
Date of publication on miami:26.10.2018
Modification date:16.04.2019
Edition statement:[Electronic ed.]
Source:BMC Genomics 18 (2017) 329, 1-14
Subjects:RNA-sequencing; Immune priming; Tribolium castaneum; Host-parasite interaction; Bacillus thuringiensis
DDC Subject:570: Biowissenschaften; Biologie
License:CC BY 4.0
Language:English
Funding:Finanziert durch den Open-Access-Publikationsfonds 2017 der Westfälischen Wilhelms-Universität Münster (WWU Münster).
Format:PDF document
URN:urn:nbn:de:hbz:6-17109691020
Permalink:http://nbn-resolving.de/urn:nbn:de:hbz:6-17109691020
Other Identifiers:DOI: 10.1186/s12864-017-3705-7
Digital documents:document%281%29.pdf

BACKGROUND: The phenomenon of immune priming, i.e. enhanced protection following a secondary exposure to a pathogen, has now been demonstrated in a wide range of invertebrate species. Despite accumulating phenotypic evidence, knowledge of its mechanistic underpinnings is currently very limited. Here we used the system of the red flour beetle, Tribolium castaneum and the insect pathogen Bacillus thuringiensis (Bt) to further our molecular understanding of the oral immune priming phenomenon. We addressed how ingestion of bacterial cues (derived from spore supernatants) of an orally pathogenic and non-pathogenic Bt strain affects gene expression upon later challenge exposure, using a whole-transcriptome sequencing approach.RESULTS: Whereas gene expression of individuals primed with the orally non-pathogenic strain showed minor changes to controls, we found that priming with the pathogenic strain induced regulation of a large set of distinct genes, many of which are known immune candidates. Intriguingly, the immune repertoire activated upon priming and subsequent challenge qualitatively differed from the one mounted upon infection with Bt without previous priming. Moreover, a large subset of priming-specific genes showed an inverse regulation compared to their regulation upon challenge only.CONCLUSIONS: Our data demonstrate that gene expression upon infection is strongly affected by previous immune priming. We hypothesise that this shift in gene expression indicates activation of a more targeted and efficient response towards a previously encountered pathogen, in anticipation of potential secondary encounter.