Quantitative Phase Imaging Using Digital Holographic Microscopy Reliably Assesses Morphology and Reflects Elastic Properties of Fibrotic Intestinal Tissue

Intestinal strictures are a frequent complication in patients with Crohn’s Disease (CD) and the presence of fibrosis within strictures impacts the therapeutic treatment approach. Here, we evaluate quantitative phase imaging (QPI) using digital holographic microscopy (DHM) for the evaluation of fibro...

Authors: Bokemeyer, Arne
Tepasse, Phil-Robin
Quill, Lena
Lenz, Philipp
Rijcken, Emile Jan Marie
Vieth, Michael
Ding, Nik
Ketelhut, Steffi
Rieder, Florian
Kemper, Björn
Bettenworth, Dominik
Division/Institute:FB 05: Medizinische Fakultät
Document types:Article
Media types:Text
Publication date:2019
Date of publication on miami:30.06.2020
Modification date:31.07.2020
Edition statement:[Electronic ed.]
Source:Scientific Reports 9 (2019) 19388, 1-11
Subjects:Biophysics; Crohn's disease; Phase-contrast microscopy
DDC Subject:610: Medizin und Gesundheit
License:CC BY 4.0
Language:English
Funding:Finanziert durch den Open-Access-Publikationsfonds der Westfälischen Wilhelms-Universität Münster (WWU Münster).
Format:PDF document
URN:urn:nbn:de:hbz:6-60189423820
Permalink:http://nbn-resolving.de/urn:nbn:de:hbz:6-60189423820
Other Identifiers:DOI: 10.1038/s41598-019-56045-2
Digital documents:artikel_kemper_2019.pdf

Intestinal strictures are a frequent complication in patients with Crohn’s Disease (CD) and the presence of fibrosis within strictures impacts the therapeutic treatment approach. Here, we evaluate quantitative phase imaging (QPI) using digital holographic microscopy (DHM) for the evaluation of fibrosis within CD strictures. 30 full thickness resection specimens were obtained from non-stenotic and stenotic tissue areas of 15 CD patients. Cryostat sections were analyzed by DHM to measure the spatial distribution of the refractive index (RI) to quantify tissue density. Complementary, histopathological evaluation of H&E staining and immunofluorescence (IF) targeting fibrosis markers served as the gold standard. Moreover, tissue stiffness was evaluated by elastography. RI values assessed by DHM were significantly higher in stenotic compared to non-stenotic tissue areas (p