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Influence of antiTNF-alpha antibody treatment on fracture healing under chronic inflammation

Background: The overexpression of tumor necrosis factor (TNF)-α leads to systemic as well as local loss of bone and cartilage and is also an important regulator during fracture healing. In this study, we investigate how TNF-α inhibition using a targeted monoclonal antibody affects fracture healing in a TNF-α driven animal model of human rheumatoid arthritis (RA) and elucidate the question whether enduring the anti TNF-α therapy after trauma is beneficial or not. Methods: A standardized femur fracture was applied to wild type and human TNF-α transgenic mice (hTNFtg mice), which develop an RA-like chronic polyarthritis. hTNFtg animals were treated with anti-TNF antibody (Infliximab) during the fracture repair. Untreated animals served as controls. Fracture healing was evaluated after 14 and 28 days of treatment by clinical assessment, biomechanical testing and histomorphometry. Results: High levels of TNF-α influence fracture healing negatively, lead to reduced cartilage and more soft tissue in the callus as well as decreased biomechanical bone stability. Blocking TNF-α in hTNFtg mice lead to similar biomechanical and histomorphometrical properties as in wild type. Conclusions: High levels of TNF-α during chronic inflammation have a negative impact on fracture healing. Our data suggest that TNF-α inhibition by an anti-TNF antibody does not interfere with fracture healing.

Titel: Influence of antiTNF-alpha antibody treatment on fracture healing under chronic inflammation
Verfasser: Timmen, Melanie GND
Hidding, Heriburg GND
Wieskötter, Britta GND
Baum, Wolfgang GND
Pap, Thomas
Raschke, Michael J.
Schett, Georg GND
Zwerina, Jochen
Stange, Claude Richard GND
Organisation: FB 13: Biologie
FB 05: Medizinische Fakultät
Dokumenttyp: Artikel
Medientyp: Text
Erscheinungsdatum: 29.05.2014
Publikation in MIAMI: 21.11.2014
Datum der letzten Änderung: 27.07.2015
Quelle: BMC Musculoskeletal Disorders 15 (2014) 184, 1-6
Schlagwörter: Anti-TNFα; Inflammation; Fracture healing; Rheumatoid arthritis; Treatment
Fachgebiete: Medizin und Gesundheit
Lizenz: CC BY 4.0
Sprache: Englisch
Anmerkungen: Finanziert durch den Open-Access-Publikationsfonds 2014/2015 der Deutschen Forschungsgemeinschaft (DFG) und der Westfälischen Wilhelms-Universität Münster (WWU Münster).
Format: PDF-Dokument
URN: urn:nbn:de:hbz:6-91339470608
Permalink: https://nbn-resolving.org/urn:nbn:de:hbz:6-91339470608
DOI: doi:10.1186/1471-2474-15-184
ISSN: 1471-2474
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