Bradykinin assay for monitoring serum protease activity

A step-by-step protocol for monitoring serum protease activity using dabsylated bradykinin is provided. Detection is performed using thin-layer chromatography. The assay can be run with as little as 3 µl of serum and responds particularly well in cases when angiotensin-converting enzyme activity is...

Authors: König, Simone
Bayer, Malte C.
Division/Institute:FB 05: Medizinische Fakultät
Document types:Article
Media types:Text
Publication date:2017
Date of publication on miami:10.10.2017
Modification date:02.11.2017
Source:Mercator Journal of Biomolecular Analysis, 1 (2017) 2, S. 1-11
Edition statement:[Electronic ed.]
Subjects:Bradykinin; neuropeptide; angiotensin-converting enzyme; dabsyl chloride; TLC; serum protease
DDC Subject:572: Biochemie
License:CC BY-SA 3.0 DE
Language:English
Format:PDF document
URN:urn:nbn:de:hbz:6-30289538583
Permalink:http://nbn-resolving.de/urn:nbn:de:hbz:6-30289538583
Digital documents:mercator-journal_2017_1_2.pdf

A step-by-step protocol for monitoring serum protease activity using dabsylated bradykinin is provided. Detection is performed using thin-layer chromatography. The assay can be run with as little as 3 µl of serum and responds particularly well in cases when angiotensin-converting enzyme activity is changed.

In diesem Artikel wird eine Laborvorschrift zur Durchführung eines Peptid-Assays zur Untersuchung der Protease-Aktivität in Serum veröffentlicht. Dieser beruht auf der Inkubation des ACE-Substrates Bradykinin in wenigen Mikrolitern Serum. Dazu wird das Peptid dabsyliert. Die Detektion der gebildeten Peptidfragmente erfolgt mittels Dünnschichtchromatographie.