Strategies and approaches in plasmidome studies—uncovering plasmid diversity disregarding of linear elements?

The term plasmid was originally coined for circular, extrachromosomal genetic elements. Today, plasmids are widely recognized not only as important factors facilitating genome restructuring but also as vehicles for the dissemination of beneficial characters within bacterial communities. Plasmid dive...

Authors: Dib, Julian Rafael
Wagenknecht, Martin
Farias, Maria E.
Meinhardt, Friedhelm
Division/Institute:FB 13: Biologie
Document types:Article
Media types:Text
Publication date:2015
Date of publication on miami:22.06.2015
Modification date:16.04.2019
Edition statement:[Electronic ed.]
Source:Frontiers in Microbiology 6 (2015) 463, 1-12
Subjects:plasmidome; circular plasmid; linear plasmid; extrachromosomal DNA; episome; metagenomics
DDC Subject:570: Biowissenschaften; Biologie
License:CC BY 4.0
Language:English
Notes:Finanziert durch den Open-Access-Publikationsfonds 2015/2016 der Westfälischen Wilhelms-Universität Münster (WWU Münster).
Format:PDF document
ISSN:1664-302X
URN:urn:nbn:de:hbz:6-59229623296
Permalink:http://nbn-resolving.de/urn:nbn:de:hbz:6-59229623296
Other Identifiers:DOI: 10.3389/fmicb.2015.00463
Digital documents:fmicb-06-00463.pdf

The term plasmid was originally coined for circular, extrachromosomal genetic elements. Today, plasmids are widely recognized not only as important factors facilitating genome restructuring but also as vehicles for the dissemination of beneficial characters within bacterial communities. Plasmid diversity has been uncovered by means of culture-dependent or -independent approaches, such as endogenous or exogenous plasmid isolation as well as PCR-based detection or transposon-aided capture, respectively. High-throughput-sequencing made possible to cover total plasmid populations in a given environment, i.e., the plasmidome, and allowed to address the quality and significance of self-replicating genetic elements. Since such efforts were and still are rather restricted to circular molecules, here we put equal emphasis on the linear plasmids which—despite their frequent occurrence in a large number of bacteria—are largely neglected in prevalent plasmidome conceptions.