%0 Article %A König, Simone %A Harrach, Saliha %A Berdel, Wolfgang E. %A Schwöppe, Christian %D 2017 %G English %T Pseudo-MRM method for the selective detection of truncated tissue factor %J Mercator Journal of Biomolecular Analysis %U https://nbn-resolving.de/urn:nbn:de:hbz:6-00269468510 %U https://repositorium.uni-muenster.de/document/miami/74aafda0-d757-4dad-bd54-8b0ee5b171cd/mercator-journal_2017_1_2_22-32.pdf %7 [Electronic ed.] %X A pseudo-MRM method using high-resolution mass spectrometry is presented for the specific detection of truncated tissue factor (tTF), retargeted to tumor vasculature by GNGRAHA peptide (tTF-NGR), in serum. In order to increase sensitivity, serum proteins are pre-separated by 1D-gel electrophoresis and the gel band corresponding to recombinant human tTF-NGR is investigated. Calibration is performed using the gelseparated pure protein. The method relies on six unique tryptic peptides and is sensitive at the low fmol level.