Erweiterte Suche

A laboratory manual of kidney perfusion techniques

An organ such as the kidney naturally functions best in a healthy organism. Once a kidney has been removed from its normal context, it is crucial to find the optimal compromise between the demands of the problem at issue and the experimental model to be adopted. An isolated kidney is, in effect, a “dying kidney”, although it may continue to function for between 1 and 4 hours, depending on one's definition of normal. The basic reason for the progressive deterioration in function can be found in the stresses set up by the transition from perfusion by whole blood to the use of a more or less artificial perfusing medium. Indeed, blood is a “liquid organ”, a highly complex cocktail that is steadily being reconditioned by the actions of other organs, and this complexity explains why all efforts to maintain kidney function in isolation are ultimately doomed to failure. If we want to approach its fantastic properties, then we should leave an organ as the kidney within the body. As Mephisto remarks in Goethe's drama “Faust”, Studierzimmer II: “Blut ist ein ganz besondrer Saft”: “blood is a quite special juice”.

Titel: A laboratory manual of kidney perfusion techniques
Verfasser: Schurek, Hans-Joachim GND
Weitere Beteiligte: Neumann, Klaus Hinrich GND
Schweda, Frank GND
Czogalla, Jan GND
Dokumenttyp: Buch
Medientyp: Text
Erscheinungsdatum: 2017
Publikation in MIAMI: 06.07.2017
Datum der letzten Änderung: 16.04.2019
Reihe Wissenschaftliche Schriften der WWU Münster / Reihe V ; 6
Verlag/Hrsg.: readbox unipress / Münster (Westf)
Schlagwörter: isoliert perfundierte Niere; Sauerstoff; Modellvarianten; Konzentrierfähigkeit
isolated perfused kidney; oxygen; different modalities; concentrating capacity
Fachgebiete: Medizin und Gesundheit
Lizenz: CC BY-SA 4.0
Sprache: Englisch
Anmerkungen: Auch im Buchhandel erhältlich: A Laboratory Manual of Kidney Perfusion Techniques / Hans-Joachim Schurek; In collaboration with Klaus Hinrich Neumann, Frank Schweda and Jan Czogalla. – Münster : Münsterscher Verlag für Wissenschaft, 2017. – VIII, 228 S. (Wissenschaftliche Schriften der WWU Münster : Reihe V ; Bd. 6), ISBN 978-3-8405-0154-8, Preis: 71,50 EUR
Deutsche Ausgabe 2015 unter dem Titel "Laborhandbuch Nierenperfusion" erschienen, http://nbn-resolving.de/urn:nbn:de:hbz:6-88219516586, https://doi.org/10.17879/67229530894
Format: PDF-Dokument
ISBN: 978-3-8405-0154-8
URN: urn:nbn:de:hbz:6-22259740573
Permalink: https://nbn-resolving.org/urn:nbn:de:hbz:6-22259740573
DOI: 10.17879/61219766654
Onlinezugriff:
Inhalt:
Preface
Table of Contents ..... V-VIII
Introduction ..... 1
1. Overview of experimental models of the kidney ..... 5
1.1. Historical aspects of the perfusion of isolated kidneys ..... 5
1.2. Oxygen supply – a critical parameter ..... 7
2. General principles of kidney perfusion ..... 8
2.1. Single-pass (once-through) perfusion ..... 9
2.2. Recirculation ..... 9
2.3. Recirculation and regeneration of the perfusate by dialysis ..... 11
2.4. Reperfusion of an anatomically perfusion-fixed kidney as a model ..... 11
3. Advantages and general limitations of in-vitro perfusion of the mammalian kidney ..... 12
3.1. Flow-constant versus pressure-constant perfusion ..... 18
3.2. Concentrating and diluting capacity ..... 18
3.3. The permeability of albumin in the IPRK and the “repaired defect” hypothesis ..... 21
4. Perfusion techniques ..... 27
4.1. Perfusion medium ..... 27
4.2. Perfusion drive systems, measurement of flow and pressure ..... 37
4.3. Temperature control ..... 41
4.4. Aeration ..... 44
4.5. Filtering ..... 54
4.6. Cannulation ..... 57
5. Material for the perfusion apparatus ..... 66
5.1. Single-pass System ..... 66
5.2. Recirculation system ..... 76
5.3. Recirculation mode with dialyzer for aeration and regeneration ..... 78
5.4. Reperfusion after anatomical fixation ..... 80
6. Sterilization and disinfection procedures ..... 83
6.1. Disinfection bath ..... 84
6.2. Thermal disinfection ..... 84
6.3. Sterilization by ethylene oxide ..... 84
6.4. On sterility of the perfusate ..... 84
7. Anesthesia ..... 86
8. Surgical techniques and connection procedure ..... 86
8.1. Selection of surgical instruments ..... 86
8.2. Surgical technique and connection procedures ..... 87
9. Measurements: Parameters and methods ..... 99
9.1. Temperature ..... 99
9.2. Perfusion flow rate ..... 100
9.3. Oxygen consumption ..... 102
9.4. Urinary flow rate, urinary flow blockage ..... 102
9.5. Glomerular filtration rate (GFR) and its measurement ..... 103
9.6. Electrolytes Na, K, Ca, Cl, HCO3 ..... 104
9.7. Osmolality and colloid-osmotic pressure ..... 106
9.8. Colloids, substrates incl. amino acids, protein and glucose ..... 109
10. Kidney perfusion: comparison of results ..... 114
10.1. Single pass perfusion and recirculation with dialysis ..... 114
10.2. Recirculation perfusion. Perfusion experiments on the endocrine function of the kidney ..... 133
10.3. Recirculation with regeneration of the perfusate by dialysis ..... 137
10.4. The anatomically fixed kidney as a tool for analyzing the glomerular filter ..... 138
11. Tabellarium and abbreviations for the tables ..... 141
12. Annex ..... 150
12.1. Biochemical studies on the energy metabolism of the IPRK ..... 150
12.2. Morphological studies of the IPRK, perfused either cell-free or with erythrocyte-containing medium ..... 153
12.3. Hypothesis to account for oxygen deficiency in the cell-free perfused kidney ..... 167
12.4. Overview of the contributions of our group to the four different techniques of renal perfusion ..... 177
(Klaus Hinrich Neumann, Göttingen)
13. Special aspects of micropuncture experiments on the IPRK ..... 180
13.1. Studies on the isolated perfused rat kidneys ..... 180
13.2. Glomerular morphometry ..... 184
(Frank Schweda, Regensburg)
14. The isolated perfused mouse kidney ..... 187
14.1. Perfusion techniques ..... 187
14.2. Perfusion medium ..... 189
14.3. Perfusion apparatus ..... 190
14.4. Surgical preparation and cannulation of the renal artery ..... 193
Ad 14.2 Appendix ..... 195
(Sheep erythrocytes as O2 carriers during perfusion of the isolated
mouse kidney. Contributed by Jan Czogalla, Zurich, Institute of
Anatomy, University of Zurich, Switzerland)
15. Technical appendix and image collection of the IPRK ..... 197
16. Appendix on the history of renal perfusion 1849–1908 ..... 208
17. References ..... 212