Extraction of δ-aminolevulinic acid-induced protoporphyrin IX from blood and serum and measurement using analytical chromatography coupled to mass spectrometry

In continuation of earlier work on the detection of δ-aminolevulinic acid (ALA)-induced protoporphyrin IX (PPIX) in blood and serum both the extraction method and the analysis were improved considerably. Liquid chromatography (LC) analysis was transferred from capillary equipment to an analytical wo...

Authors: Peters, Insa
König, Simone
Division/Institute:FB 05: Medizinische Fakultät
Document types:Article
Media types:Text
Publication date:2019
Date of publication on miami:19.12.2019
Modification date:19.12.2019
Source:Mercator Journal of Biomolecular Analysis, 3 (2019) 2, S. 6-9
Edition statement:[Electronic ed.]
Subjects:Protoporphyrin; MRM; glioblastoma; serum; chromatography Protoporphyrin; MRM; Glioblastoma; Serum; Chromatographie
DDC Subject:572: Biochemie
License:CC BY-SA 3.0 DE
Language:English
Format:PDF document
URN:urn:nbn:de:hbz:6-12189602979
Permalink:http://nbn-resolving.de/urn:nbn:de:hbz:6-12189602979
Digital documents:mercator_journal_2019_3_2_6-9.pdf

In continuation of earlier work on the detection of δ-aminolevulinic acid (ALA)-induced protoporphyrin IX (PPIX) in blood and serum both the extraction method and the analysis were improved considerably. Liquid chromatography (LC) analysis was transferred from capillary equipment to an analytical workflow reducing the experiment time from several hours to 10 min. These promising results enable LC coupling to modern mass spectrometers which boosts the sensitivity about 100-fold.

Als Fortsetzung von Arbeiten zur Bestimmung von δ-Aminolävulinsäure (ALA)-induziertem Protoporphyrin IX (PPIX) in Blut und Serum wurden sowohl die Extraktionsmethode als auch die Analysis wesentlich verbessert. Von Kapillar-Flüssigchromatographie wurde auf eine höhere Flussrate umgestellt, was die notwendige Zeit für ein Experiment von mehreren Stunden auf 10 min verkürzte. In Verbindung mit neuen Massenspektrometern ist mit dieser Methode eine ca. 100-fache Empfindlichkeitsverbesserung zu erwarten.