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Mutations of the EPHB6 Receptor Tyrosine Kinase Induce a Pro-Metastatic Phenotype in Non-Small Cell Lung Cancer

Alterations of Eph receptor tyrosine kinases are frequent events in human cancers. Genetic variations of EPHB6 have been described but the functional outcome of these alterations is unknown. The current study was conducted to screen for the occurrence and to identify functional consequences of EPHB6 mutations in non-small cell lung cancer. Here, we sequenced the entire coding region of EPHB6 in 80 non-small cell lung cancer patients and 3 tumor cell lines. Three potentially relevant mutations were identified in primary patient samples of NSCLC patients (3.8%). Two point mutations led to instable proteins. An in frame deletion mutation (del915-917) showed enhanced migration and accelerated wound healing in vitro. Furthermore, the del915-917 mutation increased the metastatic capability of NSCLC cells in an in vivo mouse model. Our results suggest that EPHB6 mutations promote metastasis in a subset of patients with non-small cell lung cancer.

Titel: Mutations of the EPHB6 Receptor Tyrosine Kinase Induce a Pro-Metastatic Phenotype in Non-Small Cell Lung Cancer
Verfasser: Bulk, Etmar Michael GND
Yu, Jun
Hascher, Antje GND
Koschmieder, Steffen GND
Wiewrodt, Rainer Gerhard GND
Krug, Utz GND
Timmermann, Bernd
Marra, Alessandro
Hillejan, Ludger GND
Müller-Tidow, Carsten GND
Organisation: FB 05: Medizinische Fakultät
Dokumenttyp: Artikel
Medientyp: Text
Erscheinungsdatum: 04.12.2012
Publikation in MIAMI: 25.02.2013
Datum der letzten Änderung: 22.08.2014
Quelle: PLOS ONE 7 (2012) 12, e44591
Fachgebiete: Medizin und Gesundheit
Lizenz: CC BY 2.5
Sprache: Englisch
Anmerkungen: Finanziert durch den Open-Access-Publikationsfonds 2012/2013 der Deutschen Forschungsgemeinschaft (DFG) und der Westfälischen Wilhelms-Universität Münster (WWU Münster).
Format: PDF-Dokument
URN: urn:nbn:de:hbz:6-37379422304
Permalink: http://nbn-resolving.de/urn:nbn:de:hbz:6-37379422304
DOI: 10.1371/journal.pone.0044591
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