Customized Chitooligosaccharide Production – Controlling the Length via Engineering of Rhizobial Chitin Synthases and the Choice of Expression System
Chitooligosaccharides (COS) can be produced by partial depolymerisation of chitin or in microbial cell factories expressing a chitin synthase (CHS) from Rhizobia. As different CHS have been reported to produce COS of different chain lengths, we here show the results of molecular dynamics simulations...
| Authors: | |
|---|---|
| Division/Institute: | FB 13: Biologie |
| Document types: | Research data |
| Media types: | Text |
| Publication date: | 2022 |
| Date of publication on miami: | 26.04.2022 |
| Modification date: | 25.08.2022 |
| Edition statement: | [Electronic ed.] |
| Subjects: | Chitooligosaccharides; COS; Chitin Synthases; Biotechnological Production; Chitin Oligomer; Protein-Engineering; Rhizobia |
| DDC Subject: | 570: Biowissenschaften; Biologie |
| License: | CC BY-NC-SA 4.0 |
| Language: | English |
| Funding: | Förderer: Ministerium für Wirtschaft, Innovation, Digitalisierung und Energie des Landes Nordrhein-Westfalen / Projektnummer: 313-W044A |
| Format: | application/octet-stream |
| URN: | urn:nbn:de:hbz:6-74089644534 |
| Other Identifiers: | DOI: 10.17879/74089643745 |
| Permalink: | https://nbn-resolving.de/urn:nbn:de:hbz:6-74089644534 |
| Digital documents: | customized_cos_production_nodc.pse |
Chitooligosaccharides (COS) can be produced by partial depolymerisation of chitin or in microbial cell factories expressing a chitin synthase (CHS) from Rhizobia. As different CHS have been reported to produce COS of different chain lengths, we here show the results of molecular dynamics simulations of two CHS with their bound COS products of different chain lengths. The PyMOL file comprises representative frames visualizing the binding of membrane embedded NodC from Rhizobium sp. GRH2 (NodC_GRH2) and NodC from Sinorhizobium (Ensifer) meliloti (NodC_Sm) to their natural products. Shown are the fully acetylated chitin tetramer (A4) and pentamer (A5) in NodC_Sm as well as pentamer (A5) and hexamer (A6) in NodC_GRH2. The designations indicate which NodC (GRH2 or Sm), which substrate (A4-A6) and which simulation (I-II) as well as which frame is depicted.