Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells

Cancer gene therapy requires the design of non-viral vectors that carry genetic material and selectively deliver it with minimal toxicity. Non-viral vectors based on cationic natural polymers can form electrostatic complexes with negatively-charged polynucleotides such as microRNAs (miRNAs). Here we...

Authors: Santos Carballal, Beatriz
Aaldering, Lukas
Ritzefeld, M.
Pereira, S.
Sewald, N.
Moerschbacher, Bruno Maria
Götte, Martin
Goycoolea, Francisco Martin
Division/Institute:FB 13: Biologie
Document types:Article
Media types:Text
Publication date:2015
Date of publication on miami:08.10.2015
Modification date:16.04.2019
Edition statement:[Electronic ed.]
Source:Scientific Reports 5 (2015) 13567, 1-15
DDC Subject:570: Biowissenschaften; Biologie
License:CC BY 4.0
Language:English
Notes:Finanziert durch den Open-Access-Publikationsfonds 2015/2016 der Westfälischen Wilhelms-Universität Münster (WWU Münster).
Format:PDF document
ISSN:2045-2322
URN:urn:nbn:de:hbz:6-78219455692
Permalink:http://nbn-resolving.de/urn:nbn:de:hbz:6-78219455692
Other Identifiers:DOI: 10.1038/srep13567
Digital documents:srep13567.pdf

Cancer gene therapy requires the design of non-viral vectors that carry genetic material and selectively deliver it with minimal toxicity. Non-viral vectors based on cationic natural polymers can form electrostatic complexes with negatively-charged polynucleotides such as microRNAs (miRNAs). Here we investigated the physicochemical/biophysical properties of chitosan–hsa-miRNA-145 (CS–miRNA) nanocomplexes and the biological responses of MCF-7 breast cancer cells cultured in vitro. Self-assembled CS–miRNA nanocomplexes were produced with a range of (+/−) charge ratios (from 0.6 to 8) using chitosans with various degrees of acetylation and molecular weight. The Z-average particle diameter of the complexes was